Quantitation of Toxoplasma gondii DNA in a competitive nested polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
Quantitation of Toxoplasma gondii DNA in a competitive nested polymerase chain reaction.
AIM To quantify Toxoplasma gondii DNA using a specially constructed artificial template as competitor in a nested polymerase chain reaction (PCR). METHODS The diagnostic assay was a nested PCR employing four primers that amplify part of the single copy gene for the P30 major surface antigen in T gondii. An artificial competitor containing the four primer binding sites was made first by creati...
متن کاملTechnical report Quantitation of Toxoplasma gondii DNA in a competitive nested polymerase chain reaction
Aim—To quantify Toxoplasma gondii DNA using a specially constructed artificial template as competitor in a nested polymerase chain reaction (PCR). Methods—The diagnostic assay was a nested PCR employing four primers that amplify part of the single copy gene for the P30 major surface antigen in T gondii. An artificial competitor containing the four primer binding sites was made first by creating...
متن کاملDetection Of Toxoplasma Gondii and Human Cytomegalovirus DNA in Blood from Transplant Recipients Using Multiplex Nested Polymerase Chain Reaction
Evidences from many studies suggested a polymerase chain reaction (PCR) as a valuable method for diagnosing infectious disease in the transplant recipients. We used this method for detection of Toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or kidney transplantation. DNA of both infectious agents were detected using two separate sets of nested pr...
متن کاملdetection of toxoplasma gondii and human cytomegalovirus dna in blood from transplant recipients using multiplex nested polymerase chain reaction
evidences from many studies suggested a polymerase chain reaction (pcr) as a valuable method for diagnosing infectious disease in the transplant recipients. we used this method for detection of toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or kidney transplantation. dna of both infectious agents were detected using two separate sets of nested pr...
متن کاملQuantitation of enteroviral RNA by competitive polymerase chain reaction.
The polymerase chain reaction (PCR) is a new diagnostic technique for the detection of enteroviral infection; however, it currently provides only qualitative results. The aim of this study was to adapt PCR for the accurate quantitation of enteroviral RNA in clinical specimens. For this purpose, we designed a standard RNA which was homologous to sequences at the 5' end of the coxsackie B3 entero...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1999
ISSN: 0021-9746
DOI: 10.1136/jcp.52.1.61